Pengaruh Variasi Waktu dan Kecepatan Centrifuge terhadap Nilai Hematokrit Metode Makrohematokrit

Authors

  • Subur Wibowo Akademi Analis Kesehatan Pekalongan
  • Isnaini Isnaini Akademi Analis Kesehatan Pekalongan

DOI:

https://doi.org/10.59744/jumeha.v4i1.63

Keywords:

Hematocrit, Centrifuge, Speed, Duration, Red Blood Cells

Abstract

Hematocrit is the volume percentage of red blood cells, which is an important parameter in the diagnosis of various health conditions. In this study, there were 9 samples with 3 repetitions. Each sample is then processed using a centrifuge with varying speeds and durations. The centrifuge speeds used included 3000, 3500, and 4000 RPM, while processing times were set at 10, 15, and 20 minutes. The results showed that there was a significant relationship between the speed and duration of the centrifuge and the hematocrit value obtained. In general, increasing centrifuge speed and processing duration tends to result in higher hematocrit values. However, at a certain rate and duration, further increases did not provide significant changes in hematocrit values, indicating the existence of a saturation point where optimal conditions have been reached. A centrifuge speed of 3000 RPM for 10 minutes was found to be the most efficient combination to obtain accurate hematocrit values ​​without damaging red blood cells. This study provides important insights for clinical laboratories in determining optimal parameters for accurate and efficient hematocrit measurements. Proper setting of centrifuge speed and duration not only increases the accuracy of results but also reduces operational time and costs. Further studies are recommended to explore the effects of other factors such as temperature and type of centrifuge used on hematocrit values.

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Published

2024-03-25

How to Cite

Subur Wibowo, & Isnaini Isnaini. (2024). Pengaruh Variasi Waktu dan Kecepatan Centrifuge terhadap Nilai Hematokrit Metode Makrohematokrit. Jurnal Medika Husada, 4(1), 25–35. https://doi.org/10.59744/jumeha.v4i1.63